Plasmid_Backbone

Part:BBa_J18966:Design

Designed by: Sean Sleight   Group: Affiliates   (2013-06-18)


pSS3K2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 1385
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XhoI site found at 178
    Illegal XhoI site found at 1021
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal AgeI site found at 1471
    Illegal AgeI site found at 1794
    Illegal AgeI site found at 4611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2499
    Illegal SapI site found at 4593


Design Notes

Inserted luxR and araC to be constitutively expressed in the reverse direction on the vector. Removed restriction sites in prefix and suffix to improve assembly success rate.


Source

Modified pGA3K3 from 2011 University of Washington iGEM team.


References